Agrobacterium Protocols by Kevan M. A. Gartland, Michael R. Davey

By Kevan M. A. Gartland, Michael R. Davey

Agrobacterium Protocols deals starting and skilled researchers the main complete choice of step by step protocols for the genetic manipulation of crops utilizing Agrobacterium. the subjects diversity from the upkeep of bacterial tradition collections to elements of the metabolism and body structure of remodeled tissues and transgenic crops. Drawing at the paintings of major scientists from laboratories all over the world, Agrobacterium Protocols presents a wealth of options for introducing particular DNA sequences into aim plant species and discusses the environmental implications of genetically engineered vegetation. Its specified strategies will facilitate fast move of complex thoughts to different laboratories and their exploitation in basic and utilized plant biology.

Show description

Read or Download Agrobacterium Protocols PDF

Similar methodology & statistics books

Regulators and Effectors of Small GTPases, Part F: Ras Family I

The significantly acclaimed laboratory typical for greater than forty years, equipment in Enzymology is among the so much hugely revered courses within the box of biochemistry. on account that 1955, every one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence includes a lot fabric nonetheless suitable this day, actually a necessary booklet for researchers in all fields of existence sciences.

The use of electrochemical scanning tunnelling microscopy (EC-STM) in corrosion analysis: Reference material and procedural guidelines (EFC 44) (European Federation of Corrosion Publications)

Using EC-STM to check the connection among floor constitution and floor reactivity in situ on electrodes in touch with an electrolyte is of significant significance in corrosion examine. Highlighting this crucial subject in corrosion technology, this publication provides the reference fabric and procedural instructions required for the appliance of electrochemical scanning tunnelling microscopy (EC-STM).

Clinical Applications of PCR (Methods in Molecular Biology)

During this up to date moment variation, prime researchers follow molecular diagnostics to the various fresh advances that experience happened in polymerase chain response( PCR)-based applied sciences. Highlights comprise real-time PCR, which permits the strategy to be played in a quantitative demeanour with enhanced sensitivity, robustness, and resilience to carryover infection, mass spectrometric research of nucleic acids, and circulating cell-free nucleic acids in plasma.

Additional info for Agrobacterium Protocols

Sample text

EMBO J. $1445-1454 9. Stachel, S E and Zambryski, P (1986) virA and virG control the plant-induced activation of the T-DNA transfer process of Agrobacterium tumefaciens. Cell 46,325-333 10. Douglas, C. , and Nester, E. W. (1982)Agrobacterium tumefaciens mutants affected m attachment to plant cells. J. Bacterial. 152, 1265-1275. 11. An, G , Ebert, P. , and Ha, S B (1988) Binary vectors, m Plant Molecular Biology Manual (Gelvin, S. B. and Schilperoort, R. ), Kluwer, Dordrecht, Netherlands, pp A3 1-19.

Bacterial. 152, 1265-1275. 11. An, G , Ebert, P. , and Ha, S B (1988) Binary vectors, m Plant Molecular Biology Manual (Gelvin, S. B. and Schilperoort, R. ), Kluwer, Dordrecht, Netherlands, pp A3 1-19. 12. Lichtenstem, C P and Fuller, S. L. (1987) Vectors for genetic engineering of plants. Genet Eng 6, 103-183. , Dhaese, P , Schreier, P. , and Schell, J (1983) Size, location and polarity of T-DNA-encoded transcripts m 58 An nopaline crown gall tumors; common transcripts in octopine and nopaline tumors.

Binary Vectors for Promoter Analysis Binary vectors can also be used for studying regulatory elements involved in transcription initiation. The promoter vectors, pGA580, pGA581, pGA582, and pGA809 (Figs. 2 and 3) were developed from a basic binary vector by inserting a promoterless cat reporter gene immediately downstream from the multiple cloning sites (II). Insertion of a promoter fragment in front of the reporter gene results in transcription of the cat mRNA. If the fragment carries only the promoter region and does not contain the coding region, the cut mRNA will produce a native chloramphenical acetyltransferase (CAT) enzyme.

Download PDF sample

Rated 4.50 of 5 – based on 8 votes