By Kevan M. A. Gartland, Michael R. Davey
Agrobacterium Protocols deals starting and skilled researchers the main complete choice of step by step protocols for the genetic manipulation of crops utilizing Agrobacterium. the subjects diversity from the upkeep of bacterial tradition collections to elements of the metabolism and body structure of remodeled tissues and transgenic crops. Drawing at the paintings of major scientists from laboratories all over the world, Agrobacterium Protocols presents a wealth of options for introducing particular DNA sequences into aim plant species and discusses the environmental implications of genetically engineered vegetation. Its specified strategies will facilitate fast move of complex thoughts to different laboratories and their exploitation in basic and utilized plant biology.
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EMBO J. $1445-1454 9. Stachel, S E and Zambryski, P (1986) virA and virG control the plant-induced activation of the T-DNA transfer process of Agrobacterium tumefaciens. Cell 46,325-333 10. Douglas, C. , and Nester, E. W. (1982)Agrobacterium tumefaciens mutants affected m attachment to plant cells. J. Bacterial. 152, 1265-1275. 11. An, G , Ebert, P. , and Ha, S B (1988) Binary vectors, m Plant Molecular Biology Manual (Gelvin, S. B. and Schilperoort, R. ), Kluwer, Dordrecht, Netherlands, pp A3 1-19.
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Binary Vectors for Promoter Analysis Binary vectors can also be used for studying regulatory elements involved in transcription initiation. The promoter vectors, pGA580, pGA581, pGA582, and pGA809 (Figs. 2 and 3) were developed from a basic binary vector by inserting a promoterless cat reporter gene immediately downstream from the multiple cloning sites (II). Insertion of a promoter fragment in front of the reporter gene results in transcription of the cat mRNA. If the fragment carries only the promoter region and does not contain the coding region, the cut mRNA will produce a native chloramphenical acetyltransferase (CAT) enzyme.